£58.00

Südwestdeutscher Verlag für Hochschulschriften Externe Kontrolle plastidärer Genexpression in Tabak

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Last 74 days · 74 data points (no recent data)

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£58.00 £55.10 £56.26 £57.42 £58.58 £59.74 £60.90 07 June 2026 25 June 2026 13 July 2026 31 July 2026 19 August 2026

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74 days 0 19 37 56 74 £58 Days at Price

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Most common price: £58 (74 days, 100.0%)

Price range: £58 - £58

Price levels: 1 different prices over 74 days

Description

Für die Entwicklung eines plastidären Induktionssystems wurden bakterielle Elemente des quorum sensing in Nicotiana tabacum (Tabak) getestet. Die regulativen Komponenten wurden von Vibrio fischeri entnommen und an die Expressionsmaschinerie der Plastiden adaptiert. Mit einem hochsensitiven β-Glucuronidase (GUS)-Assay wurde die Expressionsstärke des Induktionssystems in den transgenen Pflanzen ermittelt. Im nichtinduzierten Zustand konnte die Expression durch die Auswahl geeigneter Promotorelemente auf eine Konzentration von 0,0003% vom gesamtlöslichen Protein erheblich gesenkt werden. Eine signifikante Steigerung der GUS-Expression mit dem spezifischen Induktor wurde nicht erzielt. Mögliche Ursachen dafür werden anhand der Literatur diskutiert. Weiterhin wurde ein transientes Expressionssystem etabliert. Mittels Polyethylenglykol (PEG) wurden Tabak-Protoplasten mit verschiedenen GUS-Expressionsvektoren transformiert und reproduzierbar eine signifikante GUS-Aktivität gemessen. Probleme bei der Analyse von Plastiden-Expressionsvektoren werden aufgezeigt und Lösungsansätze diskutiert.

Product Specifications

Format
paperback
Domain
Amazon UK
Release Date
13 July 2011
Listed Since
19 July 2011

Barcode

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