Springer Essentials of Single-Cell Analysis: Concepts, Applications and Future Prospects (Series in BioEngineering)

This book provides an overview of single-cell isolation, separation, injection, lysis and dynamics analysis as well as a study of their heterogeneity using different miniaturized devices. As an important part of single-cell analysis, different techniques including electroporation, microinjection, optical trapping, optoporation, rapid electrokinetic patterning and optoelectronic tweezers are described in detail. It presents different fluidic systems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry) and their integration with sensor technology, optical and hydrodynamic stretchers etc., and demonstrates the applications of single-cell analysis in systems biology, proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics, biomedicine and drug delivery systems. It also discusses the future challenges for single-cell analysis, including the advantages and limitations. This book is enjoyable reading material while at the same time providing essential information to scientists in academia and professionals in industry working on different aspects of single-cell analysis.  Dr. Fan-Gang Tseng is a Distinguished Professor of Engineering and System Science at the National Tsing Hua University, Taiwan. Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA. From the Back Cover This book provides an overview of single-cell isolation, separation, injection, lysis and dynamics analysis as well as a study of their heterogeneity using different miniaturized devices. As an important part of single-cell analysis, different techniques including electroporation, microinjection, optical trapping, optoporation, rapid electrokinetic patterning and optoelectronic tweezers are described in detail. It presents different fluidic systems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry) and their integration with sensor technology, optical and hydrodynamic stretchers etc., and demonstrates the applications of single-cell analysis in systems biology, proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics, biomedicine and drug delivery systems. It also discusses the future challenges for single-cell analysis, including the advantages and limitations. This book is enjoyable reading material while at the same time providing essential information to scientists in academia and professionals in industry working on different aspects of single-cell analysis.  Dr. Fan-Gang Tseng is a Distinguished Professor of Engineering and System Science at the National Tsing Hua University, Taiwan. Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA. About the Author Dr. Fan-Gang Tseng received his Ph.D. degree in mechanical engineering from UCLA, USA, in 1998. He is currently a Distinguished Professor in the ESS Department as well as at the NEMS Institute, and the Deputy Director of the Biomedical Technology Research Center at NTHU. He is also an affiliated Research Fellow of Academia Sinica, Taiwan. He was elected as an ASME fellow in 2014. His research interests are in the fields of BioNEMS, Biosensors, Micro-Fluidics, Tissue Chips, and Fuel Cells. He holds 60 patents, authored 8 book chapters, and published more than 220 journal papers and 360 conference papers. Dr. Tseng currently serves on the editorial boards of Applied Sciences, Journal of Circuits and Systems, International Journal of Molecule Science, Micromachines, and Open Nanomedicine Journal. He received many awards, including National Innovation Award (twice), Outstanding Research Award (MOST and NSC, twice), First-Class Research Award (NSC), Mr. Wu, Da-Yo Memorial Award (NSC), NTHU Outstanding Research Award (2005-2015), and more than twelve best paper/poster awards from various competitions and conferences. Dr. Tuhin Subhra Santra r