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VDM Verlag 3D-Microstructured Protein Microarrays for Cancer Diagnosis

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Description
Protein microarrays have shown a powerful tool to identify cancer biomarkers due to the multiplex detection and tiny sample requirement. However, there is no single biomarker that is sensitive and specific enough to meet strict diagnostic criteria due to the diversity and variation of cancers. To this end, a combination of biomarkers is required to increase sensitivity and specificity. In this book, various silane monolayers and polymer layers were developed to functionalize 3D micro-structured glass slides to effectively immobilize proteins, and the detection parameters were optimized for sensitive biomarker detection. Under the optimized conditions, protein microarrays reach a wide dynamic range and low limit of detection to pM range, but the analytical performance of each biomarker significantly depends on surface chemistry. The implemented protein microarrays were used for the detection of biomarkers from colorectal and breast cancer sera. Results show that the combination of cancer biomarkers on their specific surface lead to remarkably increase of the positive responses. A panel of 5 biomarkers allows to specifically detect over 82% of breast cancer patients.

Product Specifications
Brand
VDM Verlag
Format
paperback
ASIN
3639712641
Category
Books > Subjects > Science, Nature & Maths > Biological Sciences > Biochemistry
Domain
Amazon UK
Publication Date
21 July 2014
Listed Since
14 August 2014

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No barcode data available

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