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GRIN Verlag Alterations of the Sister Chromatid Exchange frequency in peripheral lymphocytes caused by an Ironman triathlon
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Description
Diploma Thesis from the year 2007 in the subject Medicine - Human Genetics, grade: 1,0, University of Vienna (Institut für Ernährungswissenschaften), language: English, abstract: The aim of the present study was to investigate the effect of a triathlon (3.8 km swim, 180 km cycle, 42,2 km run) on the genomic stability of nine highly trained non- professional athletes. Therefore, the SCE assay, a relevant biological response marker for genotoxicity in human biomonitoring studies [PENDZICH et al., 1997] was performed using peripheral lymphocytes, on account of their effortless accessibility [WILKOSCY and RYNARD, 1990]. Duplicate lymphocyte cell cultures, of each participant, were incubated for 72 h (37°C, 5% CO2) according to a short-term human lymphocyte cell culture. For each participant at least 50 metaphases, containing 43-46 chromosomes were scored, to evaluate the mean SCE frequency. The number of SCEs per cell was calculated to a chromosome set of a normal diploid human cell, containing 46 chromosomes. In the present study the alteration of SCE frequency, 48 h pre- and 24 h postrace was evaluated. As an additional endpoint Top 5 HFCs (highest five absolute SCE means) were assayed. It could be demonstrated that both the total mean SCE frequency and the mean Top 5 HFC frequency (n=9) 24 h postrace were significantly decreased (*p<0.05) compared to 48 h prerace values. Considering the training status, a significant negative correlation between the relative SCE changes before and after the triathlon was observed for the cycling training per week (km) (r=-0.86; **p<0.01), the running training per week (km) (r=-0.90; *p<0.05) as well as for the weekly net exercise training time (h) (r=-0.89; *p<0.05). The relative changes of Top 5 HFCs before and after the race correlated significantly with the cycling training per week (km) (r=-0.79; *p<0.05) and with the body mass index (kg/m2) (r=-0.69; *p<0.05). These findings suggest the existence of endogenous
Key Features
Used Book in Good Condition
Product Specifications
- Brand
- GRIN Verlag
- Format
- paperback
- ASIN
- 3638910415
- Domain
- Amazon UK
- Release Date
- 20 February 2008
- Listed Since
- 18 March 2010
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