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Mutational adaptation of E. coli cells with novel molecular biosensors: Analysis of the mutational adaptation of E. coli to produce NADPH and ... molecular biosensors and cultivation systems

Microbiology

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Description

Fatty acids play an important role in production of biodiesel as well as cosmetics and health products. One of the limiting factors in the fatty acid biosynthesis is NADPH, which is required for the reduction steps in the FAB cycle. An option to overcome this problem is the engineering of NADPH overproducing E. coli strains. Siedler et al. developed a biosensor based on the transcriptional regulator SoxR for NADPH-consuming enzymes. The activation of SoxR by high NADPH levels leads to the transcription of the SoxRS regulon. This regulon can contain many different genes and therefore can be designed to select for NADPH overproducing strains. In order to achieve this, the long-term mutational adaption of the engineered E. coli cells was tested in an ALE experiment. Through Next Generation sequencing of the genomes, a mutation in the ygcW gene was found, which could lead to a higher NADPH production.

Product Specifications

Format: paperback
ASIN: 3330521031
Category: Books > Subjects > Science, Nature & Maths > Biological Sciences > Microbiology
Domain: Amazon UK
Release Date: 18 July 2017
Listed Since: 13 August 2017

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Mutational adaptation of E. coli cells with novel molecular biosensors: Analysis of the mutational adaptation of E. coli to produce NADPH and ... molecular biosensors and cultivation systems

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