Humana Basic Cell Culture Protocols: 290 (Methods in Molecular Biology, 290)

In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side-population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis). Review "This book is written by experienced authors who understand the practical needs of bench scientists. All laboratory personnel who perform cell culture on a regular basis will benefit from it. The authors provide summaries, keywords, helpful introductions, lists of necessary materials and equipment, and detailed protocols for culturing a wide-variety of cell types. The chapters on manipulating pluripotent stem cells will be of great value to researchers interested in regenerative medicine and cell engineering. This book is an important and valuable contribution to the field of cell biology and the art of manipulating cells in culture."-Doody's Health Sciences Book Review Journal From Reviews of the Second Edition. . . "...a highly prized collection that no cell culture laboratory should be without, so much so that it is likely to be 'borrowed' and never returned!" -Retinoids & Lipid Soluble Vitamins in Clinical Practice "...any laboratory involved in cell culture will find this book of use..." -Microbiology Today "...would be most useful to have in the laboratory itself...and a valuable reference resource in a library." Four Stars -Doody's Health Sciences Book Review Journal "...an excellent starting point for those who are interested in learning the basics of cell culture and the types of manipulations that make them useful." -Journal of Chemical Neuroanatomy From the Back Cover In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of the major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis). The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Up-to-date and highly practical, Basic Cell Culture Protocols, Third Edition, offers basic scientists and clinician-researchers powerful tools to isolate, culture, and characterize the promising specialized cell types in demand today.