Humana Pluripotent Stem Cells: Methods and Protocols: 997 (Methods in Molecular Biology, 997)

Part I. Generation of Pluripotent Stem Cells 1. Sources and Derivation of Human Embryonic Stem Cells Michal Amit 2. A New Chemical Approach to the Efficient Generation of Mouse Embryonic Stem Cells Hossein Baharvand and Seyedeh-Nafiseh Hassani 3. A Review of the Methods for Human iPSC Derivation Nasir Malik and Mahendra S. Rao 4. Generation of Human Induced Pluripotent Stem Cells by Lentiviral Transduction Jennifer C. Moore 5. Generation of Induced Pluripotent Stem Cells with CytoTune, a Non-Integrating Sendai Virus Pauline T. Lieu, Andrew Fontes, Mohan C. Vemuri, and Chad C. MacArthur 6. Generation of Human Induced Pluripotent Stem Cells (hiPSCs) using Episomal Vectors on Defined Essential 8TM Medium Conditions Andrew Fontes, Chad C. MacArthur, Pauline T. Lieu, and Mohan C. Vemuri 7. Feeder-free Substrates for Pluripotent Stem Cell Culture Alexandria Sams and Mark J. Powers Part II. Culture and Differentiation of Pluripotent Stem Cells 8. Methods for Culturing Human Embryonic Stem Cells on Feeders Jasmeet Kaur and Mary Lynn Tilkins 9. Methods for Culturing Human Embryonic Stem Cells in a Xeno-Free System Jasmeet Kaur, Mary Lynn Tilkins, and Shayne Boucher 10. Directed Differentiation of Human Pluripotent Stem Cells Along the Pancreatic Endocrine Lineage Dennis Van Hoof and Muluye E. Liku 11. Directed Differentiation of Pluripotent Stem Cells to Functional Hepatocytes Philip Roelandt, Jolien Vanhove, and Catherine Verfaillie 12. Highly Efficient Directed Differentiation of Human Induced Pluripotent Stem Cells into Cardiomyocytes Paul W. Burridge and Elias T. Zambidis 13. Generation of iPSC Lines from Human Normal and Neoplastic Blood Cells Using Episomal Vectors Kejin Hu and Igor Slukvin Part III. Pluripotent Stem Cell Characterization 14. Cellular Characterization of Human Pluripotent Stem Cells Rene H. Quintanilla Jr. 15. TaqMan® OpenArray® High Throughput Transcriptional Analysis of Human Embryonic and Induced Pluripotent Stem Cells Sunali N. Patel, Yalei Wu, Yun Bao, Ricardo Mancebo, Janice Au-Young, and Elena Grigorenko 16. Epigenome Analysis of Pluripotent Stem Cells Christopher L. Ricupero, Mavis R. Swerdel, and Ronald P. Hart 17. Single Cell Gene Expression Analysis of Pluripotent Stem Cells Ronald V. Abruzzese and Richard A. Fekete 18. Profiling Stem Cells Using Quantitative PCR Protein Assays David Ruff and Pauline Lieu Part IV. Pluripotent Stem Cell Modification and Applications 19. Labeled Stem Cells as Disease Models and in Drug Discovery Catharina Ellerström, Raimund Strehl, and Johan Hyllner 20. Cloning Technologies Andrew Fontes 21. Stable Transfection Using Episomal Vectors to Create Modified Human Embryonic Stem Cells Yin Liu, Kate Wagner, and Uma Lakshmipathy 22. Site-Specific Integration in Human ESC Using Jump-In™ TI™ Technology Chad C. MacArthur Review From the reviews: “This book is a collection of methods and protocols for the successful generation and characterization of pluripotent stem cells. … It will be helpful to researchers, lab technicians, and students interested in creating pluripotent cells and their commitment to specific cell lineages. … The combination of approachable methods and extremely detailed protocols make this a comprehensive guide for anyone interested in this specific field of research. … the figures, tables, and diagrams clarify the text and make the protocols easy to understand.” (Michele Fornaro, Doody’s Book Reviews, November, 2013)“It is particularly wellcome a book devoted to the basic of the pluripotent stem cells … . All of the chapters are of great value and help in the every day lab working of the stem cell byologist, no doubt … .” (CarloAlberto Redi, European Journal of Histochemistry, Vol. 57, 2013) From the Back Cover Human pluripotent stem cells such as human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) with their unique de