Humana Dendritic Cell Protocols: 595 (Methods in Molecular Biology, 595)

This updated book presents protocols from experts in the field that cover the basics and more complex forays into the exploration of DC development and function, both in mice and humans. It aims to become a bench-side handbook for both beginners and experts. Review From the reviews of the second edition:“Written for principal investigators and their students, postdocs, and staff, providing very practical step-by-step instructions on how to work with DC. … Overall, this book provides a nice compilation of methods for studying dendritic cells. The book will be particularly useful for those studying murine DC, but will also provide some useful … information for those initiating human DC work. … this updated book, which is unique in the field, will make a nice addition to personal and institutional biomedical research libraries.” (Patricia Fitzgerald-Bocarsly, Doody’s Review Service, July, 2010) From the Back Cover Given the vital importance of immune system research, the gathering of clear, consistent, and informative protocols involving the study of dendritic cells is paramount. Bringing the popular first edition fully up to date, Dendritic Cell Protocols, Second Edition presents protocols from experts in the field that cover the basics and more complex forays into the exploration of DC development and function, both in mice and humans. The first section of the volume involving humans explores topics such as the isolation of blood DC subtypes, primary skin Langerhans cells, and the generation of gene-manipulated human DCs with the inclusion of more clinically relevant methods as well, while the second section involving rodent models delves into DC and precursor generation in vitro, isolation ex vivo, disease models, as well as DC functions and properties. Written in the highly successful Methods in Molecular Biology™ series style, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Dendritic Cell Protocols, Second Edition aims to become a bench-side handbook for both beginners and experts in the field of DC research and a long-term reference for some of the most popular methods put forward by those who lead the field.