Humana Epitope Mapping Protocols: 524 (Methods in Molecular Biology, 524)

Expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping. Review From the reviews of the second edition: "This is a beautifully presented and very well-written book on the subject … . The diagrams and data are of a very high standard and black and white … . a fantastic lab resource and would benefit any immunology lab where epitope mapping is being or will be used. … a useful reference book of interest to staff from Pls to PhD students, providing both a thorough background and working methods. … a high quality and reader friendly book and I would highly recommend it." (Barbara Guinn, Immunology News, August, 2009) From the Back Cover Given the versatile utility of the determinination of epitopes, beneficial to a wide variety of scientists from immunologists to structural biologists to biotechnologists, the need for a thorough, state-of-the-art collection of experimental protocols is clear.  In Epitope Mapping Protocols, Second Edition, expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping as well as a new section on the profiling of antibody signatures in biological fluids. Written in the popular Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Epitope Mapping Protocols, Second Edition is a reliable and valuable reference for all those who wish to understand and further investigate the diversifying field of epitope mapping.