Humana Germline Stem Cells: 450 (Methods in Molecular Biology, 450)

In this comprehensive and cutting-edge book, leading experts explore the parameters that define germline stem cells and the mechanisms that regulate the cell behavior in order to better isolate, characterize and maintain them. The volume begins by providing protocols for germline stem cell identification and regulation in model organisms, and concludes with detailed chapters covering current techniques involving in vitro culture and the applications of the cells. Review From the reviews: "This book provides background information and protocols for identifying, isolating, characterizing, and manipulating … germline stem cells. … This book is written for basic science and clinical researchers … as well as those interested in development and reproductive biology. Biomedical scientists and clinicians … will also appreciate the wealth of new information. … This is an outstanding contribution to the literature in germ cell biology. ... will be valued by all those interested in development of the germ lineage and stem cell biology." (Bruce A. Fenderson, Doody’s Review Service, October, 2008) From the Back Cover The knowledge gained by studying germline stem cells, the source of human and animal reproduction, may find immediate application in preserving endangered wildlife, managing commercial livestock, overcoming fertility problems in humans, and treating testicular and ovary tumors.  In Germline Stem Cells, leading experts explore the parameters that define germline stem cells and the mechanisms that regulate the cell behavior in order to better isolate, characterize and maintain them.  Divided into two parts, the volume begins by providing protocols for germline stem cell identification and regulation in model organisms, and concludes with detailed chapters covering current techniques involving in vitro culture and the applications of the cells.  Following the highly successful Methods in Molecular Biology™ series format, each chapter includes a brief introduction to the subject, a list of necessary equipment and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Germline Stem Cells is the perfect reference for scientists exploring this particular exciting and important subdivision in the fast-paced field of stem cell research.