Humana Electroporation Protocols: Preclinical and Clinical Gene Medicine: 423 (Methods in Molecular Biology, 423)

In this volume, top researchers provide hands-on protocols describing the process of non-viral delivery of genetic materials such as DNA in order to better treat disease. Each method and protocol offers step-by-step, readily reproducible laboratory instructions, with the benefit of several introductory reviews to aid investigators new to this exciting technology. Notes on troubleshooting and avoiding known pitfalls are helpfully provided. From the Back Cover At the time of the lightbulb's invention, not even the most imaginative thinkers could have predicted the prevention and treatment of disease through genes delivered by electric fields.  In Electroporation Protocols: Preclinical and Clinical Gene Medicine, top researchers provide hands-on protocols describing the process of non-viral delivery of genetic materials such as DNA, oligo-DNA, and RNA into targeted immune cells, tissues, and model animals in order to better treat disease.  Following the successful Methods in Molecular Biology™ series format, each method and protocol offers step-by-step, readily reproducible laboratory instructions, with the benefit of several introductory reviews to aid investigators new to this exciting technology. Ideal for beginners and essential for the more knowledgable, Electroporation Protocols: Preclinical and Clinical Gene Medicine collects a comprehensive set of the newest discoveries and applications in a revolutionary field that offers so much to our fight against disease.