Humana Apoptosis and Cancer: Methods and Protocols: 414 (Methods in Molecular Biology, 414)

The aim of Apoptosis and Cancer is to describe the performance of contemporary techniques for studying the biology of apoptosis and its role in cancer. The protocols described will aid both the academic laboratory interested in further characterizing the mechanisms of apoptosis, as well as the industry laboratory, aimed at identifying new target molecules or screening for new compounds with potential clinical use. Review From the reviews: "Divided among 17 chapters, the condensed information about apoptosis, its pathways and the performance of progressive techniques for apoptosis assessment are presented. … The valuable and most appreciated part of each chapter are notes – the reader will find here the plethora of hints, the real grain of wisdom, virtual wizard that saves a lot of struggling time at benchtop. This book belongs to the category of must-have books." (J. Sedlák, Neoplasma, September, 2008) From the Back Cover Our understanding of the apoptotic pathway and its regulation has improved significantly in the last 10 years. However, as our knowledge of the intricate intracellular events increase, we realize the complexity of the regulatory mechanisms that control it. As such, Apoptosis and Cancer describes the performance of contemporary techniques for studying the biology of apoptosis and its role in cancer. This book is a collaboration between academics- and industry-based scientists. The chapters have the technical development characteristic of an academic environment and the standardized system necessary for translating a product to general use, as provided by the industry. Thus, the protocols described within this groundbreaking new volume will aid both an academic laboratory interested in further characterizing the mechanism of apoptosis and an industry laboratory interested on identifying new target molecules or screening for new compounds with potential clinical use. The authors – all leaders in the field – have created a comprehensive text that covers the newest available techniques as well as conventional basic techniques. Apoptosis and Cancer will undoubtedly provide the necessary resources to everyone interested in the area of apoptosis.