Humana Stem Cells in Regenerative Medicine: Methods and Protocols: 482 (Methods in Molecular Biology, 482)

As regenerative medicine involves replacing diseased cells, tissues or organs, or repairing tissues in vivo, the manipulation of stem cells underlie its goals. In Stem Cells in Regenerative Medicine: Methods and Protocols, leading experts in the field provide an updated representation of the landscape of stem cell-based therapies in a wide spectrum of tissue systems and ontogenic stages, from the isolation and culture of stem cells to their actual use in vivo. Written in the highly successful Methods in Molecular Biology™ series format, these chapters include brief introductions to the topic, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Comprehensive and easy-to-use, Stem Cells in Regenerative Medicine: Methods and Protocols is certain to contribute greatly to the definition of standardized procedures for the manipulation of somatic and embryonic stem cells in research and clinical applications. Review "Presently, early detection and treatment remain our best hope for the cure of this common disease. Dr. Klein has assembled an outstanding group of authors to contribute to Management of Prostate Cancer. Those who take the time to read it will have a much better understanding of the management of prostate cancer."-Foreword by Ralph A. Straffon, MD ., ."Klein's Management of Prostate Cancer provides a balanced and comprehensive review...the 20 chapters are well written and provide a balanced view of the issues in diagnosis and treatment. The book's best feature is that it presents both sides of the picture...Thompson and Foley present in a point-counterpoint style arguments that support or refute the effectiveness of screening, the clinical significance of detected disease, and the effectiveness of local treatment, as well as opposing views on cost and the quality of data from prospective trials...A second feature of this book is its practical approach to the management of prostate cancer and the complications of treatment...The third feature of this book is its emphasis on new horizons...Management of Prostate Cancer is an informative review, and most of the chapters should be readily understood by patients and physicians alike. The last two chapters, on the surgeon's and radiation oncologist's perspectives, would be valuable reading for any man with prostate cancer who must decide whether to undergo surgery or radiation therapy. The chapter on screening provides important insights for primary care physicians facing the problem of whether to recommend screening."-The New England Journal of Medicine "This book is a must read for any physiciandesiring a complete and up-to-date understanding of prostate cancer. All aspects of this variable, complex, and prevalent disease are discussed in the 20 chapters, with contributions from 43 clinicians involved in the diagnosis and treatment of prostate cancer...Outstanding discussions analyze risk factors and genetic markers...The Management of Prostate Cancer will provide the clinician with a current, complete snapshot of all issues involved in the diagnosis and management of this disease. It should be part of the library of all urologists. Pathologists, radiation oncologists, medical oncologists, surgical oncologists, and general surgeons will find this text very useful as a reference for the latest developments in this field."-Current Surgery "The book offers recent survey in the field of prostate cancer epidemiology, risk factor, molecular markers, diagnostic procedures (including prostate biopsy), transrectal ultrasonography, computer tomography, magnetic resonance, the techniques of radical prostatectomy, radiotherapy and hormone therapy. Moreover there are on clinical practice oriented chapters, as interpretation of pathological findings, perioperative care, life quality evaluation, posttherapeutical urological disorders and modes of sexual