Humana Neurodegeneration: Methods and Protocols: 793 (Methods in Molecular Biology, 793)

Part I. Neurodegeneration 1. Introduction to Neurodegenerative Diseases and Related Techniques Hibiki Kawamata and Giovanni Manfredi 2. In vivo and In vitro Determination of Cell Death Markers in Neurons Nicole Naniche, Daniela Sua, and Piera Pasinelli 3. Degenerating Processes Identified by Electron Microscopic Immunocytochemical Methods Teresa A. Milner, Elizabeth M. Waters, Danielle C. Robinson, and Joseph P. Pierce Part II. Neural Cell Culture Techniques 4. Isolation and Culture of Neurons and Astrocytes from the Mouse Brain Cortex Hyun-Jeong Kim and Jordi Magrané 5. Isolation and Culture of Postnatal Spinal Motoneurons Carol Milligan and David Gifondorwa 6. Converting Human Pluripotent Stem Cells to Neural Tissue and Neurons to Model Neurodegeneration Stuart M. Chambers, Yvonne Mica, Lorenz Studer, and Mark J. Tomishima 7. Neural Differentiation of Induced Pluripotent Stem Cells Mark Denham and Mirella Dottori Part III. Models of Neurodegeneration 8. Developing Yeast Models of Human Neurodegenerative Disorders Alejandro Ocampo and Antoni Barrientos 9. Modeling Dopamine Neuron Degeneration in C. elegans Michelle L. Tucci, Adam J. Harrington, Guy A. Caldwell, and Kim A. Caldwell 10. Role of the Proteasome in Fly Models of Neurodegeneration Chun-Hung Yeh, Marlon Jansen, and Thomas Schmidt-Glenewinkel 11. Modeling Neurodegenerative Diseases in Zebrafish Embryos Angela Laird and Wim Robberecht 12. Practical Considerations of Genetic Rodent Models for Neurodegenerative Diseases Kindiya Geghman and Chenjian Li 13. Modeling Focal Cerebral Ischemia In vivo Katherine Jackman, Alexander Kunz, and Costantino Iadecola 14. Pharmacological Models of Parkison's Disease in Rodents Peter Klivenyi and Laszlo Vecsei 15. Behavioral Phenotyping of Mouse Models of Neurodegeneration Magali Dumont Part IV. Neurodegenerative Disease Mechanisms - Proteinopathies 16. Characterization of Amyloid Deposits in Neurodegenerative Diseases Ruben Vidal and Bernardino Ghetti 17. Detection of Protein Aggregates in Neurodegenerative Diseases Han-xiang Deng, Eileen H. Bigio, and Teepu Siddique 18. Assessment of Proteasome Impairment and Accumulation/Aggregation of Ubiquitinated Proteins in Neuronal Cultures Natura Myeku, Maria Jose Metcalfe, Qian Huang, and Maria Figueiredo-Pereira Part V. Neurodegenerative Disease Mechanisms - Mitochondria 19. Measurements of Threshold of Mitochondrial Transition Pore Opening in Intact and Permeabilized Cells by Flash Photolysis of Caged Calcium Andrey Y. Abramov and Michael R. Duchen 20. Isolation and Functional Assessment of Mitochondria from Small Amounts of Mouse Brain Tissue Christos Chinopoulos, Steven F. Zhang, Bobby Thomas, Vadim Ten, and Anatoly A. Starkov 21. Monitoring Mitophagy in Neuronal Cell Cultures Jianhui Zhu, Ruben K. Dagda, and Charleen T. Chu Part VI. Neurodegenerative Disease Mechanisms - Biological functions 22. Analysis of Vesicular Trafficking in Primary Neurons by Live Imaging Davide Tampellini and Gunnar K. Gouras 23. Surface Trafficking of Sodium Channels in Cells and in Hippocampal Slices Doo Yeon Kim and Dora M. Kovacs 24. Imaging Pr Review From the reviews: “The authors describe various cell and molecular systems related to neurodegeneration research. … there are a lot of protocols and methods described by the book that can be used by medical students, graduate students, junior, and senior investigators. I heartily recommend this book to university and research institute communities working on neurodegeneration and regeneration.” (Joseph J. Grenier, Amazon.com, September, 2013)“This is an authoritative collection of manuscripts tailored to bench scientists involved in neurodegenerative research. … It is intended for cell biologists, molecular biologists, biochemists, pathologists, and researchers in the field of neurodegeneration. … This book is most appropria