Humana Pancreatic Cancer: Methods and Protocols: 980 (Methods in Molecular Biology, 980)

1. Identification and Analysis of Precursors to Invasive Pancreatic Cancer Hanno Matthaei, Marco Dal Molin, Anirban Maitra, 2. Tissue Microarrays: Construction and Use Helen Remotti 3. Immunohistochemistry of Pancreatic Neoplasia Sukhwinder Kaur, Tomohiro Shimizu, Michael J. Baine, Sushil Kumar and Surinder K. Batra 4. A Method for Conducting Highly Sensitive microRNA in situ Hybridization and Immunohistochemical Analysis in Pancreatic Cancer Lorenzo F. Sempere, and Murray Korc 5. Optimal Molecular Profiling of Tissue and Tissue Components: Defining the Best Processing and Microdissection Methods for Biomedical Applications Jaime Rodriguez-Canales, Jeffrey C. Hanson, Jason D. Hipp, Ulysses J. Balis, Michael A. Tangrea, Michael R. Emmert-Buck, and G. Steven Bova 6. Considerations for Sequencing Analyses of Pancreatic Cancer Progression and Metastasis Alvin Makohon-Moore and Christine A. Iacobuzio-Donahue 7. DNA Methylation Analysis in Human Cancer Eileen O'Sullivan, Michael Goggins 8. Quantitative Real-Time PCR Expression Analysis of Peripheral Blood Mononuclear Cells in Pancreatic Cancer Patients Michael J. Baine, Kavita Mallya, and Surinder K. Batra 9. Development of a Cytokine-modified Allogeneic Whole Cell Pancreatic Cancer Vaccine Dan Laheru, Barbara Biedrzycki, and Elizabeth M. Jaffee 10. The Prevention and Genetics of Pancreatic Cancer - A Programmatic Approach Aimee L Lucas, Michelle M Chang, Marla D Lipsyc, and Harold Frucht 11. Development of Orthotopic Pancreatic Tumor Mouse Models Wanglong Qiu, Gloria H. Su 12. Evaluating Dietary Compounds in Pancreatic Cancer Modeling Systems Emman Mascariñas, Guido Eibl , Paul J. Grippo 13. Quantification of Murine Pancreatic Tumors by High Resolution Ultrasound Stephen A. Sastra and Kenneth P. Olive 14. Microdissection and Culture of Murine Pancreatic Ductal Epithelial Cells Yuliya Pylayeva-Gupta, Kyoung Eun Lee and Dafna Bar-Sagi 15. Sphere-forming Assays for Assessment of Benign and Malignant Pancreatic Stem Cells Julia Wang, Jennifer M. Bailey, Meritxell Rovira, Steven D. Leach 16. Analysis of Transplanted Bone Marrow Derived Cells in Chronic Pancreatitis Christoph B. Westphalen, Frederic Marrache, Timothy C. Wang 17. Analysis of the Potential for Pancreatic Cancer Metastasis In Vitro and In Vivo Chen Huang and Keping Xie 18. The use of Fluorescent Probes in the Study of Reactive Oxygen Species in Pancreatic Cancer Cells Richard D. Dinnen, Yuehua Mao, and Robert L. Fine 19. Analysis of Tumor Associated Mucin Glycotopes by Western Transfer Methods Vinayaga S. Gnanapragassam, Maneesh Jain, Satyanaraya Rachagani, Moorthy P. Ponnusamy, and Surinder K Batra 20. Evaluating Tumor Angiogenesis Minji Kim, Jessica Kandel, Jan Kitajewski 21. Pooled Genome-wide RNAi Screenings Ruth Rodriguez-Barrueco, Netonia Marshall, Jose M. Silva 22. Pooled ShRNA screenings: Computational Analysis Jiyang Yu, Preeti Putcha, Andrea Califano, Jose M. Silva 4. A Method for Conducting Highly Sensitive microRNA in situ Hybridization and Immunohistochemical Analysis in Pancreatic Cancer Lorenzo F. Sempere, and Murray Korc 5. Optimal Molecular Profiling of Tissue and Tissue Components: Defining the Best Processing and Microdissection Methods for Biomedical Applications Jaime Rodriguez-Canales, Jeffrey C. Hanson, Jason D. Hipp, Ulysses J. Balis, Michael A. Tangrea, Michael R. Emmert-Buck, and G. Steven Bova 6. Considerat Review From the reviews of the second edition:“This book describes methods and techniques that are used in pancreatic cancer research. … It will be most useful for postdoctoral researchers and novice principal investigators, as well as any investigators who want to focus on pancreatic cancer research, particularly if they are looking for a technique to fit a specific need. … It is a useful reference and go-to guide for exploring new protocols and techniques. It is also handy for seeing how an exis