Humana Plant Metabolomics: Methods and Protocols: 860 (Methods in Molecular Biology, 860)

Estimating the metabolite complement of plant material involves a wide, and growing, range of techniques. This up-to-date guide provides stepwise, reproducible methodologies as well as key notes and implementation advice from experts in the field. Review From the reviews: “The chapters presented in this volume stay true to the Methods and Protocols series, with a short piece of background information, a highly detailed step-by-step protocol, and additional practical information that will allow beginners in the field to set up repeatable, high-quality metabolomics experiments. … this is an excellent addition to the series and can provide muchneeded support, especially to scientists starting in the growing field of plant metabolomics.” (Alexandre Foito, Annals of Botany, Vol. 113 (7), April, 2014)“The book ‘Plant Metabolomics. Methods and Protocols’ is fresh in ideas and a useful manual for scientists, researchers, scholars, students and the wider readership interested in plant metabolism and metabolite molecules. … The purpose of this volume is to provide information on basic practical questions concerning experimental work with metabolomics. … The book is a mix between a scientific treatise and a laboratory manual. As such it is an excellent one.” (Tadeusz Aniszewski, Plant Science Bulletin, Vol. 58 (4), 2012) From the Back Cover Estimation of the metabolite complement of plant material involves a wide range of techniques and technologies and that breadth continues to increase.  Metabolomics research typically involves multiple sites for material preparation and analysis and most investigations are “high throughput”, meaning that chemical analysis of sample sets are inevitably carried out over an extended period of time.  In, Plant Metabolomics: Methods and Protocols expert researchers in the field detail many of the stages which are now commonly used to study plant metabolomics workflow. Stages of this workflow, up to and including the statistical analysis, accurate and detailed collection of meta-data are also essential for good process management, to satisfy reporting requirements and to ensure wider interpretability and reuse  results.Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.       Through and intuitive Plant Metabolomics: Methods and Protocols,  seeks to aid scientists in the further study of the methods for all the stages of the plant metabolomics workflow.