Humana In Vitro Mutagenesis Protocols: Third Edition: 634 (Methods in Molecular Biology, 634)

The third edition of In Vitro Mutagenesis Protocols represents a practical toolbox containing protocols vital to advancing our understanding of the connection between nucleotide sequence and sequence function. Review From the reviews of the third edition:“This well-illustrated book details specialty tools successfully employed to unravel the intricacies of protein-protein interaction, protein-structure function, protein regulation of biological processes, and protein activity and mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. … Scientists, students, and others interested in the field of in vitro mutagenesis will find this extremely useful. … This book successfully describes a variety of in vitro mutagenesis protocols for scientists working in this area.” (Omer Iqbal, Doody’s Review Service, November, 2010) From the Back Cover In the post-genomic era, in vitro mutagenesis has emerged as a critically important tool for establishing the functions of components of the proteome. The third edition of In Vitro Mutagenesis Protocols represents a practical toolbox containing protocols vital to advancing our understanding of the connection between nucleotide sequence and sequence function. Fully updated from the previous editions, this volume contains a variety of specialty tools successfully employed to unravel the intricacies of protein-protein interaction, protein structure-function, protein regulation of biological processes, and protein activity, as well as a novel section on mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, In Vitro Mutagenesis Protocols, Third Edition offers today's researchers a valuable compendium of reliable and powerful techniques with which to illuminate the proteome and its rich web of biological implications.