Wiley-Blackwell Functional Imaging by Controlled Nonlinear Optical Phenomena (Wiley Series in Biomedical Engineering and Multi–Disciplinary Integrated Systems)

Ultrafast lasers allow high-precision imaging and manipulation for biological and medical applications. Nonlinear optical microscopy has provided researchers with unique possibilities of three-dimensional imaging of biological cells and tissues. Nonlinear optical imaging technique is a rapidly emerging research area with widespread fundamental research and clinical applications. Nonlinear optical imaging allows both structural and functional imaging with cellular level resolution imaging in biological systems. The introduction of endogenous or exogenous probes can selectively enhance contrast for molecular targets in a living cell as well as supply functional information on processes. With the aim to control nonlinear optical processes and to obtain functional images, nonlinear optical processes can be controlled by photo-controlled probes and/or parameters of ultrafast laser pulses, such as time, space, polarization, and phase. This book gives an overview of the nonlinear optical process by ultrafast laser pulses and explains how the basics of nonlinear optical microscopy led to the most advanced techniques of photo-controlled nonlinear optical microscopy. Review "The authors have provided a remarkable reference onnonlinear optical and functional imaging, which includes powerfulmicroscopy and spectroscopy techniques via ultrafast laser pulses .. . Researchers and graduate students interested in microscopy, spectroscopy and nonlinear optics would benefit from thisbook." (Optics & Photonics News, 4 April2014) From the Back Cover A comprehensive guide to theories, techniques, andtechnologies for the functional imaging of biological systems usingnonlinear microscopy Nonlinear optical imaging using ultrafast laser pulses is apowerful microscopy technique that has become a valued researchtool within the biological sciences. Allowing for veryhigh-precision imaging and manipulation of cells, nonlinear opticalimaging is revolutionizing our understanding of functionalprocesses in living tissue, and the number of research and clinicalapplications for it continues to grow. Written by a team of distinguished experts from the Universityof Osaka, this is a comprehensive guide to state-of-the-artnonlinear microscopy of biological systems using ultrafast laserpulses. The authors review the evolution of nonlinear opticalmicroscopy, from its origins in the 1990s to today's most advancedphoto-controlled techniques, and explore an array of vital topics, including: Conventional nonlinear optical microscopy Ultrafast laser optics required for nonlinear opticalmicroscopy Ultrafast laser manipulation in biological and biomedicalresearch Functional imaging based on molecular control The full range of current nonlinear optical microscopictechniques, from two-photon absorption microscopy to nonresonantfour-wave mixing microscopy An array of biological applications, including nanosurgery andtransfection Providing balanced coverage of both theory and practice, Functional Imaging by Controlled Nonlinear Optical Phenomenais an indispensable primer for cell biologists, life scientists, and biomedical researchers, engineers, and technicians. About the Author KEISUKE ISOBE, DEng, is a Research Scientist at the RIKEN Center for Advanced Photonics. WATARU WATANABE, DEng, is a Professor in the Department of Electrical & Electronic Engineering, College of Science and Engineering, at Ritsumeikan University. KAZUYOSHI ITOH, DEng, is a Professor Emeritus and Research Professor at the Science Technology Entrepreneurship Laboratory (E-Square), Osaka University.