Clinical Applications of Capillary Electrophoresis: 27 (Methods in Molecular Medicine, 27) - Image 1

£41.64

Humana Clinical Applications of Capillary Electrophoresis: 27 (Methods in Molecular Medicine, 27)

Name: Clinical Applications of Capillary Electrophoresis: 27 (Methods in Molecular Medicine, 27)
Brand: Humana
Price: 41.64 GBP
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Laboratory Medicine

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Description

The term "electrophoresis" was first used by Michaelis in 1909, to - scribe the migration of colloids in an electric field. The first practical elect- phoresis method was described by Tiselius in 1937. He used a U-tube filled with buffer layered on top of sample; migration could be monitored using Schlieren optics. In zone electrophoresis, the U-tube was replaced by paper, a support material employed simply to prevent or minimize diffusion of ions, so that ions applied in a narrow strip to the paper will separate and remain as relatively discrete zones. Paper was superceded by a variety of other media, - cluding cellulose acetate, hydrolyzed starch (starch gel), agarose, and polyacry- mide. The latter, in addition to being a support medium, has size-sieving properties. From the basic zone electrophoresis, other means of separation have been dev- oped. These include, isoelectric focusing, isotachophoresis, density gradient el- trophoresis, and various forms of immunoelectrophoresis. In some ways Capillary Electrophoresis (CE) has gone full circle back to the original method of Tiselius. In its simplest form, separations occur in a buffer solution within a glass (fused silica) tube and detection occurs as sample moves past an optical window. CE has rapidly developed into a technique that rivals HPLC in its versatility. All the classical electrophoretic separations-zone, IEF, and isotachophoresis-have their counterparts in CE. Excitingly so, and - thoritatively treated in Clinical Applications of Capillary Electrophoresis.

Product Specifications

Brand: Humana
Format: hardcover
ASIN: 0896036391
Category: Books > Subjects > Health, Family & Lifestyle > Medical & Healthcare Practitioners > Allied Health Professions > Laboratory Medicine
Domain: Amazon UK
Release Date: 01 June 1999
Listed Since: 02 January 2007

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Humana Clinical Applications of Capillary Electrophoresis: 27 (Methods in Molecular Medicine, 27)

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